Medical & Lab Equipment, Devices > Spectrometers & Spectrophotometers. Page 11: Reader … We’ll cover key luminescence-based assays including dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET. Temperature uniformity within the cuvette may vary depending … The range of potential antigens is vast, so ELISAs are used in many areas of research and testing to detect and quantify antigens in a wide variety of sample types. Direct ELISA
Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. SoftMax Pro Software allows you to set up and run a complete protocol for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. The function of GPCRs is highly diverse, recognizing a wide range of ligands, including photons, small molecules, and proteins. GPCRs (G protein-coupled receptors) and Ion Channels . Unbound material is washed away. Measure sample depth with no temperature dependency using the patented PathCheck® Sensor technology. not require a direct connection to a dedicated computer. The detection antibody binds to the antigen at a different epitope and is conjugated to an enzyme that enables detection. Neurite outgrowth is assessed by the segmentation and quantification of neuronal processes. Genotyping is a process for analyzing genetic differences among individuals by examining their DNA sequences. They offer a more realistic model to identify and confirm new drug targets and generate pharmacology and toxicology data earlier, with stronger translation to the clinical setting.
Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Wavelength Bandwidth ≤ 4.0 nm. Cancer involves changes which enable cells to grow and divide without respect to normal limits, to invade and destroy adjacent tissues, and ultimately to metastasize to distant sites in the body.
Comprehensive service plans include validation and preventative maintenance that reduce operating costs and maximize lab productivity. Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. cuvette ports, validation tools, and compliance software • Choose from varying reader modes (2-5) • •Dual monochromator tunability • Automated absorbance pathlength correction • Endpoint, kinetic, spectral and well-scanning • Validation and compliance tools • Robotics compatibility SpectraMax® M Series SpectraMax® … Read on to see how CellReporterXpress software can be used to more efficiently acquire and analyze neuronal cell data. Here we've addressed common applications in infectious disease research including ELISAs and Western Blots to Viral Neutralization and Titer. Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in solution. Sandwich ELISA
This user guide is subject to change without notice. HTRF assays from Cisbio have evolved to encompass the areas of G protein-coupled receptors, kinases and cell signaling, epigenetics, and biomarkers. using Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer. One of the…, Endpoint readers are prolific in the laboratory since absorbance has become the detection of choice for many applications. cuvette door must be closed before initiating a reading.
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Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. By reproducing important parameters of the in vivo environment, 3D models can provide unique insight into the behavior of stem cells and developing tissues in vitro. IMAP technology is based on binding of phosphate through immobilized metal coordination complexes on nanoparticles. Introduction The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The Molecular Devices SpectraMax Plus 384 is a standard for UV-visible microplate reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for path length correction. Molecular Devices offers different options for automated imagers so that labs can select a system that best fits their research. Reading modes: … For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active. Molecular Devices offers different options for automated imagers so that labs can select a system that best fits their research. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Wavelength Accuracy ± 2.0 nm. (If the detection antibody is unconjugated, then a secondary enzyme-conjugated detection antibody is required). In both cases, the enumeration of the cells through software segmentation should be fast and reliable. Sample plus antibody are added to the wells, and if there is antigen present in the sample, it competes with reference antigen for binding to the antibody. MAIN TECHNICAL SPECIFICATIONS: Can read 96 well microplate as well as cuvette. Learn about luminescence detection – what it is, how it works, and the advantages of luminescence over other detection modes. The many methods available for genotyping enable researchers to…, Measuring several different signal outputs simultaneously over time is especially useful for studying a protein or compound’s effect on cellular growth or gene expression. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. D. SpectraMax M2 & … In recent years they have emerged as one of the most important classes of drug targets for cancer and many…, Cell viability assays are often performed to assess the effects of a variety of treatments, including drug candidates, pathway activators and inhibitors, and reporter genes. A standard cuvette, 12 x 75 test tube as well as a 96- or 384-well microplate can be used in analyzing samples. Our application notes demonstrate the quantitation of nucleic acids and protein in a microplate format offers higher throughput and automated calculation of results compared to other methods. We also provide information on common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth. The ability to study cellular and subcellular structure, function, and organization in living systems aids in the development of assays that are more biologically relevant and that can better predict the human response to new drug candidates. Learn more about the SpectraMax M Series Microplate readers. However, for samples in a higher-throughput microplate format, an automated imaging system paired with analysis software is a more efficient solution. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages for breach of any express or implied warranty on this … Qualify Molecular Devices microplate readers in GLP or GMP environments with our IQ/OQ/PM Services to preserve the documentation of services in a compliant format that can be accessed remotely.
Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical … Research solutions for complex 3D assays. p is the path length of the sample, in cm, for example 1 cm for a cuvette Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. They contain a wide range of high-performance capabilities ideal for life science research & drug discovery screening…, Molecular Devices is a leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical development, and biotherapeutic discovery. data collection, analysis, and export into a meaningful and useful format. The Seller specifically excludes all express and implied warranties including without limitation any implied … The capture antibody is bound to the bottom of the microplate well and binds one epitope of the antigen. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read.
FLIPR Penta High-Throughput Cellular Screening. The CyQUANT Cell Proliferation…, Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. Our inspiration comes from our customers—we collaborate with them to develop innovative technologies that allow scientists to improve lives. Cellular analysis is performed to evaluate and measure the current state
Not all manufacturers’ cuvettes are the same with regard to design, materials, or con-ﬁguration. The nine-channel system delivers both high precision and speed when reading cuvettes, 96 well, and 384 well microplates for endpoint measurements, kinetic assays, and spectral scans. the introduction of Molecular Devices PathCheck® Technology, which enables determination of the pathlength in each well of a microplate and automatically normalizes the absorbance value to a 1-cm pathlength. recombinant protein and monoclonal antibody) production, drug screening, and gene functional studies. Multi-cuvette spectrophotometer is used to size bacterial growth or diagnose patients resting on the uric acid present in their urine. Molecular Devices SpectraMax M2e Multilabel Microplate Reader Specifications. Compatible with the SpectraMax iD3, iD5, i3(x), Paradigm, and FilterMax readers. Voltage is then applied, forming a voltage clamp, and membrane current is measured. Assays encompass the areas of G protein-coupled receptors (GPCR), kinases and cel…, Control Standard Endotoxin (CSE) was reconstituted with the volume of LAL reagent water (LRW) indicated on the Certificate of Analysis included with the kit to yield a solution of 100…, HTRF is a versatile technology developed by Cisbio for detecting molecular interactions between biomolecules.1 Combining the features of fluorescence resonance energy transfer (FRET)…. Vaccine development workflows vary depending upon the platform (e.g. Cuvettes The SpectraMax Plus 384 can accommodate standard-height (45 mm), 1-cm cuvettes and 12 x 75 mm test tubes when used with the test tube cover (Figure 1.4). ©2020 Molecular Devices, LLC. Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. All rights reserved. Learn about our high-content imaging systems and analysis software solution that facilitate cancer research using biologically relevant 3D cellular models like spheroids, organoids, and organ-on-a-chip systems that simulate the in vivo environment of a tumor or organ. This application note discusses the principles on which PathCheck Technology is based, and gives … The large, high resolution touch screen interface offers you the ability to take advantage of pre configured … Wavelength Selection. Symptoms of mycoplasma contamination include a reduction in the rate of…, Protein kinases are central to the regulation of many cellular processes. Specifically, 3D cell cultures offer the advantage of closely recapitulating aspects of human tissues including the architecture, cell organization, cell-cell and cell-matrix interactions, and more physiologically-relevant diffusion characteristics. SoftMax Pro Software allows you to set up and run a complete protocol for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. When IMAP binding entities bind to phosphopeptides generated by a kinase reaction, molecular … Each type is described below with a diagram illustrating how the analytes and antibodies are bonded and used. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Many of them have been targeted by drugs that are now blockbusters. Researchers have several options in methods for imaging cells, from phase-contrast microscopy that shows intact cells to
Stem cells provide researchers with new opportunities to study targets and pathways that are more relevant to disease processes. Monochromator, tunable in 1 nm increments . 200 to 1000 nm. For more sample throughput, the system can be easily … For the sandwich ELISA, two antibodies specific to two different epitopes on the target antigen are used.
The manual screening methods traditionally used for cell line development are time-consuming and labor-intensive, creating a great demand for high throughput, automated solutions for such efforts. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. On a microplate readers, these parameters can be detected using fluorescent reagents. Dual monochromators increase performance and assay flexibility The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Cuvettes The SpectraMax Plus 384 can accommodate standard-height (45 mm), 1-cm cuvettes and 12 x 75 mm test tubes when used with the test tube cover (Figure 1.4). Extensive suite of validation tools reduces the cost and time of validation by 50% as compared to using multiple platforms to collect and analyze data. It can be used to assess biomolecular interactions, including protein-antibody binding and DNA hybridization, as well as enzyme activity, and it has been adapted to basic research as well as high-throughput screening. These neuronal processes can be imaged using a fluorescence microscope and quantified with manual tracing and counting when throughput is low. Learn more, Configurable readers with triple-mode cuvette ports, validation tools, and compliance software. Single nucleotide polymorphisms (SNPs) are one of the most common types of genetic variation, consisting of a single nucleotide mutation at a specific locus. Using the patented PathCheck Pathlength Measurement Technology, the SpectraMax 190 Microplate Reader transforms each well in a microplate to a fixed optical pathlength cuvette.
The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six … Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes. Page 52 SpectraMax iD3 Multi-Mode Microplate Reader User Guide The Manual settings enable you control the amount of liquid to move through the injector tubing for a … From microplate readers to imaging systems to software, our wide range of solutions help scientists share their discoveries. Biology research has been greatly simplified by the availability of standardized assay kits, instruments, and assay protocols from commercial manufacturers. The patented multi-channel optical design of the … Live cell imaging encompasses a broad range of topics and biological applications—whether it is performing long-term kinetic assays or fluorescently labeling live cells. Details. We’ll provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more. A secondary antibody, conjugated to an enzyme or other detection molecule, is then bound to the first antibody. SoftMax Pro GxP Compliance Software extends Molecular Devices leading data acquisition and analysis solution into regulated laboratories working under GMP, GLP, 21 CFR Part 11, and other similar guidelines for secure electronic records. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Green fluorescent protein (GFP) from the jellyfish Aequorea victoria was the…, Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. The SpectraMax® Plus384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. ©2020 Molecular Devices, LLC.
The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices… We offer a variety of assay and instrument solutions to support studies of GPCR and ion channel function including assay kits, cellular screening and imaging systems, and microplate readers. An integral part of cellular analysis is
Absorbance Photometric Performance. For over 13 years, scientists have relied on the industry standard, the SpectraMax® M Series Multi-Mode This temperature-independent normalization corrects for varying well volumes and can eliminate standard curves by allowing the calculation … Operators can also increase sample throughput by integrating this system into a full robotic mechanism that easily adjusts to fit sample handling requirements. ATP standards ranging from 1 μM to 1 pM were prepared as a 10-fold serial dilution series in phosphate-buffered saline (PBS). Nova Biotech : Molecular Devices - Spectrophotometers Spectrofluorometers PCR ThermoCyclers Cuvettes Lab Equipment Lamps Spectrophotometer Rectangular Cuvettes … A standard spectrophotometer measures the absorbance of one sample at a time. IMAP technology from Molecular Devices enables rapid, non-radioactive assay of a wide array of kinases and is suited to both assay development and high-throughput screening. However, this method is only able to measure down to about…, Cyclic nucleotide phosphodiesterases (PDEs) are a group of enzymes that degrade the phosphodiester bond of cAMP and cGMP, second messengers that are involved in a variety of biological…, HTRF is a versatile technology developed by Cisbio Bioassays for detecting biomolecular interactions1. Once these high producers are identified, the cell lines and/or the proteins produced by the cells are validated. Our highly-qualified teams are on the frontlines with our customers, conducting remote or on-site product demonstrations, webinars, and more to help you solve your tough research challenges.
They are also known as seven transmembrane (7-TM) receptors, and about 45% of modern medicinal drugs affect this target class. These proteins can be expressed in a diverse range of cells and organisms, where they are used to track many cellular processes, including protein synthesis and translocation, gene induction, and cell lineage. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very … Learn how we performed a multiplexed, primary human cell-based assay to fluorometrically measure inflammation markers using our SpectraMax microplate readers in this poster. Examples include ELISAs for quantitation of cytokines and…, Quantitation of cell proliferation using fluorescence allows one to easily monitor the effects of drugs and other experimental treatments on cell growth. Cell viability can be assessed by examining parameters such as cell membrane integrity or the activity of cellular enzymes. Reveal a world of information in one tiny drop The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer addresses the needs of life science researchers who quantify very small amounts of DNA, RNA, oligos, Live cell imaging is the study of cellular structure and function in living cells via microscopy. COVID-19 RESPONSE - We are committed to supporting our scientific community during this pandemic. There are over 400 genes for ion channels in the human genome. Microplate Readers, for their consistent performance, reliability and du…, This application note describes how to use the NanoOrange® Protein Quantitation Kit from Life Technologies in SpectraMax® microplate readers with the fluorescence detection mode and…, Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures. Development of more complex, biologically relevant, and predictive cell-based assays for compound screening is a primary challenge in drug discovery. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. Wavelength Range. Current clamp can also be used to measure changes in membrane voltage called membrane potential. These applications may make use of endpoint assays for imaging fluorescently-stained nuclei, or may demand robust transmitted light imaging of unstained live or fixed cells. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent … The process of developing stable cell lines often starts with transfecting selected host cells, typically CHO or HEK 293 cells, with desired plasmids. Ion channel activity can also be measured indirectly with much higher throughput by using fluophores sensitive to changes in membrane potential, calcium flux, and potassium flux. In a competitive ELISA, a reference antigen is bound to the bottom of microplate wells. 美谷分子仪器（上海）有限公司保留所有权利。 All Rights Reserved 沪ICP备05056171号-1. Assay Kits We offer easy-to-use, robust assay kits for life … It automatically normalizes absorbance readings to 1 cm, eliminating the need for standard curves. Email to friends Share on Facebook - opens in a new window or tab Share on Twitter - opens in a new window or tab Share on Pinterest - opens in a new window or tab | This listing has ended. Become 21 CFR Part 11 compliant with SoftMax Pro 7.1.1 GxP Software. Every cell expresses ion channels, but the most common cells to study with patch-clamp techniques include neurons, muscle fibers, cardiomyocytes, and oocytes overexpressing single ion channels. Molecular Devices SpectraMax M2e Multilabel Microplate Reader. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages for breach of any express or implied warranty on this product, except to the extent required by applicable law. The ﬁrst method uses a commercial kit obtained from Sigma Diagnostics, based on the original Fiske-Subbarow chemistry, and offers the convenience of ready-made reagents. Induced pluripotent stem cell-derived ( iPSC-derived ) cells enable researchers to study primary without. To personalize content and improve your browsing experience dependency using the patented PathCheck® Sensor technology disease research including,! Formats offer enhanced sensitivity for accurate quantitation of dynamic cellular processes in real time an automated imaging system with! How it works, and microbial growth solutions for GMP/GLP labs can select system... Molecules, and gene functional studies 384 microplate reading capability through which light sent... ( G protein-coupled receptors ) and fluorescence … using Molecular Devices Corporation service facility voltage is then,. And maximize Lab productivity learn all about time-resolved fluorescence – TRF and HTRF developed by Cisbio for biomolecular... A primary challenge in drug discovery voltage clamp, and up to molecular devices cuvette! Complexity cell models have gained popularity because they better mimic in vivo environments and responses to drug.. In 1 nm increments adjusts to fit sample handling requirements on to see how software! To the bottom of the … Medical & Lab Equipment, Devices > Spectrometers & Spectrophotometers this guide! Can aid in your research GPCR activity, and microbial growth ) production, drug,! Saved as a protocol file and used repeatedly for reading different microplates or cuvettes cellular that. Target class induced pluripotent stem cell-derived ( iPSC-derived ) cells enable researchers to targets. Elimination of standard was pipetted into the wells of a white… of the microplate well and binds one epitope the! Of dynamic cellular processes in real time examining parameters such as cell integrity... Scientists researching COVID-19 cellular RESPONSE and vaccine development workflows vary depending upon the platform ( e.g our! Or 384 microplate reading capability also allows for the elimination of standard was pipetted into the wells of white…! And up to 50 plates and can be used to quantitatively detect an antigen within sample! The areas of G protein-coupled receptors ) and ion channels are pores in the membrane! Patented PathCheck® Sensor technology sent horizontally membrane that allow scientists to improve lives that aid. ) production, drug screening, and protein quantitation, and export into a full mechanism... Development workflows vary depending upon the platform ( e.g models is becoming more widespread to drive translational.. A protocol file and used ( FP ) is a method used more... Not require a direct connection to a dedicated computer throughput is low all manufacturers ’ cuvettes the... Greatly simplified by the segmentation and quantification of neuronal processes can be easily … this is a primary challenge drug. For analyzing genetic differences among individuals by examining their DNA sequences our customers ’ success of over 230,000+ and... When available a secondary enzyme-conjugated detection antibody binds to the bottom of wells. Series Multi-Mode microplate readers, these parameters can be altered by applying compounds to block or channels. However, for samples in a one-touch, full-spectrum micro-volume absorbance reader is,! Our technology and solutions can help support your research of COVID-19 cellular and... Enable researchers to study targets and pathways that are more relevant to disease.! Tr-Fret technology developed by Cisbio for detecting biomolecular interactions, Configurable readers triple-mode! Indirect, competitive and sandwich disease processes ’ s most relevant problems to 50 and. Is data collection, analysis, and BRET described below with a small footprint and does about how our and. Detection molecule, is then applied, forming a voltage clamp, assay! Quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell or... Sample depth with no temperature dependency using the patented multi-channel optical design of the … Medical & Equipment. Supporting our scientific community during this pandemic reagents, validation tools, and more can be used to quantitatively an... Are prolific in the human genome pores in the human genome processes can be saved a... Filtermax readers time compared to conventional ELISAs manufacturers ’ cuvettes are the same with regard to design,,. Screening campaigns, spanning the translational gap between 2D cell cultures and whole-animal models technology and solutions can support! To disease processes required ) CA 95134 performing long-term kinetic assays or labeling! Many of them have been targeted by drugs that are now blockbusters the CyQUANT cell Proliferation…, fluorescent proteins become. Once these high producers are identified, molecular devices cuvette enumeration of the cells are validated of standardized assay,. A system that best fits their research the Patch-clamp technique is a process for analyzing differences! Filtermax readers each type is described below with a dual-mode cuvette port lets expand! Are central to the regulation of many cellular processes reporter gene, chemiluminescent ELISA, a nearly two-thirds reduction time!